Abstract
A growing emphasis on using cell-based assays for compound screening and enzyme activity is fueled by the need to decrease costs, increase success rates, and identify failures during discovery and preclinical development. Moreover, the growth in cell-based screening has led to the development of novel cell lines and technologies to increase the throughput and data output of cell-based assays. Therefore, we have also developed a new reporter system that allows monitoring of HIV-1 protease activity and susceptibility to protease inhibitors in living cells.
Highlights
national Meeting of The Institute of Human Virology Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. [link 'here' using 'a href' to: http://www.biomedcentral.com/content/pdf/1742-4690-2-S1
The growth in cell-based screening has led to the development of novel cell lines and technologies to increase the throughput and data output of cell-based assays
The optimized cleavage assay is currently being used for testing current protease inhibitors to compare the IC50 values to reference virologic assays
Summary
Cell-based Assay for Testing Susceptibility of HIV-1 to Protease Rita Nogueira*‡, Sofia Coelho and João Gonçalves Address: Faculty of Pharmacy of Lisbon, Centro de Patogénese Molecular, 1600 Lisbon, Portugal Email: Rita Nogueira* - rita@ff.ul.pt * Corresponding author ‡Presenting author from 2005 International Meeting of The Institute of Human Virology Baltimore, USA, 29 August – 2 September 2005
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
