Abstract
Investigating embryonic development is necessary for improving assisted reproductive technology (ART) in humans; however, due to ethical, religious, and political complexities surrounding the use of human embryos, we have adopted the rhesus monkey as a preclinical model. Although the rhesus monkey model does not offer a limitless number of oocytes as does the rodent, it is the closest animal model for examining and understanding many aspects of human reproduction, including gamete maturation, embryonic development, embryo transfer, and implantation. The rhesus model also allows access to good quality blastocysts. The aims of this study were [1] to determine the cell number and cell allocation to the trophectoderm (TE) and inner cell mass (ICM) in rhesus blastocysts, and [2] to examine the proportion of cells undergoing DNA fragmentation in in vitro fertilization (IVF) compared to intracytoplasmic sperm injection (ICSI)-fertilized rhesus blastocysts.
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