Celastrus orbiculatus extract inhibits the migration and invasion of human glioblastoma cells in vitro.

Hao Gu,Feng Jin,Lin Yang,Songhua Lu,Jun Feng,Jue Chen,Youyang Shi,Haibo Wang,Yayun Qian,Yangqing Liu

doi:10.1186/s12906-016-1232-8

Hao Gu, Feng Jin + Show 8 more

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https://doi.org/10.1186/s12906-016-1232-8

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Abstract

BackgroundGliomas are highly aggressive tumors of the nervous system, and current treatments fail to improve patient survival. To identify substances that can be used as treatments for gliomas, we examined the effect of Celastrus orbiculatus extract (COE) on the invasion and migration of human glioblastoma U87 and U251 cells in vitro. MethodsThe effects of COE on cell viability and adhesion were tested using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cell adhesion assay, respectively. The effects of COE on cell migration and invasion were assessed by a wound-healing assay and transwell migration and invasion assays. The effects of COE on the expression of epithelial-mesenchymal transition (EMT)-related proteins and matrix metalloproteinases (MMPs) were evaluated using western blot and gelatin zymography, respectively. Finally, the effect of COE on actin assembly was observed using phalloidin-tetramethylrhodamine isothiocyanate labeling and confocal laser scanning microscopy.ResultsWe found that COE inhibited the adhesion, migration, and invasion of U87 and U251 cells in a dose-dependent manner. COE reduced N-cadherin and vimentin expression, increased E-cadherin expression, and reduced MMP-2 and MMP-9 expression in U87 and U251 cells. Furthermore, COE inhibited actin assembly in U87 and U251 cells.ConclusionsCOE attenuates EMT, MMP expression, and actin assembly in human glioblastoma cells, thereby inhibiting their adhesion, migration, and invasion in vitro.

Highlights

Gliomas are highly aggressive tumors of the nervous system, and current treatments fail to improve patient survival
Effect of Celastrus orbiculatus extract (COE) on viability of human glioblastoma cells Control U87 and U251 cells exhibited active growth in vitro, whereas cells treated with varying concentrations of COE for 24, 48, or 72 h showed significantly inhibited growth (Fig. 1; P < 0.01)
Effect of COE on invasion and migration of human glioblastoma cells In the wound-healing assay, COE treatment for 24 h significantly inhibited the migration of U87 and U251 cells in a dose-dependent manner

Summary

Introduction

Gliomas are highly aggressive tumors of the nervous system, and current treatments fail to improve patient survival. To identify substances that can be used as treatments for gliomas, we examined the effect of Celastrus orbiculatus extract (COE) on the invasion and migration of human glioblastoma U87 and U251 cells in vitro. Because gliomas are highly aggressive, they represent 80 % of all malignant tumors of the human nervous system and are associated with a high rate of recurrence [1]. The current treatments do not completely remove tumor cells generated by the glioblastoma, invasion and migration major leading death [3]. It is imperative to develop drugs that can effectively inhibit the invasion of glioblastoma cells to improve patient survival. Using human glioblastoma U87 and U251 cell lines, we examined the effects of COE on the invasion and migration of glioblastoma cells and their underlying molecular mechanisms

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