Abstract
We have examined the effects of celastrol on the proliferation, migration, and apoptosis of colon cancer HT29 cells and miR-133b as a possible mechanism of action. The HT-29 cells were incubated with celastrol at different concentrations (0, 2, 4 μmol/L) for 24 h. Cell counting kit-8, scratch wound, and flow cytometry assays were used to evaluate the effects of celastrol on the HT29 cells’ proliferation, migration, and apoptosis. The content of the miR-133b in the HT29 cells and human normal colorectal mucosa fetal human cells was measured by quantitative real-time polymerase chain reaction. After treatment with different concentrations of celastrol, the proliferation and migration of the HT29 cells were both significantly inhibited in a dose-dependent manner (P < 0.05). Additionally, flow cytometry assay showed that celastrol induced the apoptosis of the HT29 cells in a dose-dependent manner. The miR-133b in the HT-29 cells was significantly lower than that in the fetal human cells. After treatment with celastrol, the miR-133b in the HT-29 cells was 3.78 times (2 μmol/L celastrol) and 4.59 times (4μmol/L celastrol) the content of untreated cells. Celastrol significantly inhibited the proliferation and migration, and promoted apoptosis of the colon cancer cells, which may be mediated by upregulation of the miR-133b. The expression of the miR-133b in the colon cancer cells was significantly inhibited.
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