Cefotaxime Combined Ellagic Acid in a Liposomal Form for More Stable and Antimicrobial Effective Formula

Abstract

The aim of this study is loading of cefotaxime (CXM) and ellagic acid (EA) in a liposomal formula to enhance CXM corneal permeability, stability and antimicrobial activity, thin film hydration method used to form CXM- EA liposomes, particle size, zeta potential, scan electron microscope image, CXM release, drug stability and antimicrobial activity were tested. CTX entrapped in CXM – EA liposomes was 42.1 ± 3.2%, ellagic acid content was 72.1 ± 3.1%, particle size was 251.7 ± 1.2 nm, and Zeta potential was 12.4 ± 3.1 mV with a polydispersity index of 0.34 ± 0.21.In concern to CXM released, it was a dramatic rapid diffusion of raw CXM (~88%) after 1 hour, however, CXM released from CXM –EA liposomes (~50 %), after 2 h, raw CXM was completely dissolved in the buffer medium, but it takes about 8 h to be completely released to the buffered medium. Stability study was carried out among 14 days at room and refrigerator temperatures, raw CXM was expired after 7 days while the formulated CXM content was (~93 %) and (~96 %) for room and refrigerator temperature respectively, finally, antimicrobial activity was carried out against two gram positive and two gram negative microorganisms, data revealed that ellagic acid potentiates the antimicrobial activity of CXM.