Abstract
This review provides an update of the state-of-the-art of CE-MS for metabolomic purposes, covering the scientific literature from July 2008 to June 2010. This review describes the different analytical aspects with respect to non-targeted and targeted metabolomics and the new technological developments used in CE-MS for metabolomics. The applicability of CE-MS in metabolomics research is illustrated by examples of the analysis of biomedical and clinical samples, and for bacterial and plant extracts. The relevant papers on CE-MS for metabolomics are comprehensively summarized in a table, including, e.g. information on sample type and pretreatment, and MS detection mode. Future considerations such as challenges for large-scale and (quantitative) clinical metabolomics studies and the use of sheathless interfacing and different ionization techniques are discussed.
Highlights
The ultimate aim of metabolomics research is to effectively address a specific biological or clinical problem [1]
In this paper, which is a follow-up of a previous review paper covering the years 2012–2014 (Electrophoresis 2015, 36, 212–224), recent CE–MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined
The largest number of metabolites was detected by RP LC–MS, while CE–MS allowed the selective analysis of amino acids in breast milk
Summary
The ultimate aim of metabolomics research is to effectively address a specific biological or clinical problem [1] To achieve this goal, state-of-the-art analytical separation techniques are increasingly used for the global profiling of (endogenous) metabolites in biological samples [2]. It is of interest to note that CE–MS has been used for the global and reproducible profiling of native peptides and (endogenous) metabolites in a clinical setting for more than a decade [7,8,9,10]. This paper, which is a follow-up of our previous CE–MS-based metabolomics reviews [21,22,23,24], provides an overview of CE–MS approaches and strategies for metabolomics studies as reported over the past 2 years. The reader is referred to more dedicated literature for an overview concerning these topics [25,26,27,28]
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