Abstract

Circular dichroism (CD) spectroscopy is a highly used method for the examination and characterization of proteins, including, amongst other features, their secondary and tertiary structures, thermal stability, comparisons of wildtype and mutant proteins, and monitoring the binding of small molecules, folding/unfolding pathways, and formation of macromolecular complexes. This article describes CDtoolX, a new, user‐friendly, free‐to‐download‐and‐use software program that enables processing, displaying, archiving, calibrating, comparisons, and analyses of CD and synchrotron radiation circular dichroism spectroscopic data.

Highlights

  • Circular dichroism (CD) spectroscopy is a highly used method for biophysical characterizations of a wide range of biological molecules, including proteins, nucleic acids, peptides, and sugars

  • Unlike commercial CD instruments, the usage of different formats has not been as common on SRCD instruments, since most of these were developed after the introduction of the CDtool .gen format and so most have this as their main or optional output/input format.[3]

  • Each spectrum is displayed in a different color, with the cognate CD and HT spectra for the same sample depicted in the same color

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Summary

Introduction

Circular dichroism (CD) spectroscopy is a highly used method for biophysical characterizations of a wide range of biological molecules, including proteins, nucleic acids, peptides, and sugars. Both lab-based CD instruments and instruments which use synchrotron radiation as their light sources (SRCDs)[1] produce information on optical features (mostly in the ultraviolet or vacuum ultraviolet wavelength ranges) which can provide conformational information. In 2004, we created a downloadable software package (CDtool)[2] for processing and analysis of CD spectroscopic data with inputs in a range of different formats. Unlike commercial CD instruments, the usage of different formats has not been as common on SRCD instruments, since most of these were developed after the introduction of the CDtool .gen format and so most have this as their main or optional output/input format.[3]

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