基于水溶性CdTe量子点-绿原酸-血管紧张素I相互作用的荧光可逆调控

Abstract

Using mercaptopropinic acid (MPA) as the stabilizer, CdTe quantum dots (QDs) were synthesized in aqueous solution. Their particle sizes and morphology were characterized by transmission electron microscope (TEM) and atomic force microscope (AFM). Chlorogenic acid (CHA) can quench the fluorescence of CdTe QDs effectively, and the quenching values of fluorescence were proportional to the concentration of CHA in a certain concentration range. The interaction between CdTe QDs and CHA was investigated via fluorescence spectrometry, UV-Vis absorption spectrometry and Fourier transform infrared spectrometry. The preliminary judgement of the quenching mechanism is dynamic quenching, and a theoretical basis of fluorescent spectrometry methods for the determination of trace amounts of CHA was provided. When angiotensinⅠhuman acetate salt hydrate (AngⅠ) was added into the CdTe QDs-CHA system, the fluorescence of CdTe QDs was recovered in a certain concentration range, thus reversible control of fluorescence of the QDs was realized. The process of fluorescent quenching and recovering of CdTe QDs has a guiding significance for the design of fluorescence sensor and the study of mechanism of reversible control of fluorescence.