Abstract
It was found that the mixing of CdTe semiconductor nanocrystals (NCs) with luminol in the presence of KMnO 4 can induce a great sensitized effect on chemiluminescence (CL) emission. When the concentration of luminol, KMnO 4 and NaOH were fixed at 1 μM, 1 μM and 0.05 M, respectively, the most excellent performance can be obtained for the CdTe NCs sensitized CL. By means of CL and photoluminescence spectra, we suppose the enhanced CL signals resulted from the accelerated luminol CL induced by the oxidized species of CdTe NCs. Based on the finding, using thioglycolic acid-capped CdTe NCs as label and immunoglobulin G (IgG) as a model analyte, a CL immunoassay protocol for IgG content detection was developed. The strong inhibition effect of phenol compounds on luminol–KMnO 4–CdTe NCs CL system was also observed. All these findings demonstrated the possibility of semiconductor nanocrystals induced chemiluminescence to be utilized for more practical applications.
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