Abstract
Fagus grandifolia is being decimated by the fungal-insect disease complex, beech bark disease (BBD). Little to no genomic sequence makes it difficult to isolate the mechanisms of resistance. In order to conserve the rapidly declining American beech populations, quantitative PCR (qPCR) can be used to identify expression-level differences in protective compounds increased in trees resistant to BBD. Chalcone synthase and expansin have been suggested to play a role in disease resistance. Preliminary microarray experiments have shown elevated mRNA for both genes in trees resistant to BBD. Seven primers were designed for qPCR analysis to allow these protective compounds to be more fully evaluated as components of BBD resistance.
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