CDNA Cloning, Genomic Organization, and Chromosomal Localization of a Novel Human Gene That Encodes a Kinesin-Related Protein Highly Similar to Mouse Kif3C

Abstract

We report the cloning and characterization of a novel human kinesin-like gene with strong homology to the mouse kinesinKif3c.The full-length cDNA contains an open reading frame of 2382 nucleotides encoding a predicted 793 amino acid peptide that includes a 389 amino acid motor domain conserved among other kinesins. PCR and DNA sequence analysis of PAC clones containing the humanKIF3Csequence revealed that the gene contains 8 exons. All introns have the conserved GT and AG dinucleotides present at their donor and acceptor sites, respectively. We have localizedKIF3Cto chromosome band 2p23 by fluorescence in situ hybridization.