Abstract

AXenopus laevisoocyte cDNA library was screened with a PCR-generatedX. laevisvitellogenin (VTG) receptor probe and a 3.6 kb cDNA clone containing the entire open reading frame, and 5′ and 3′ noncoding regions were isolated. The deduced amino acid sequence was 72% homologous to the chicken VLDL/VTG receptor, and the characteristic domains were highly conserved. Ligand binding analysis confirmed that the cloned receptor wasXenopusVTG-specific. Although Northern blotting analysis revealed that this gene was expressed as a major transcript of 3.6 kb inXenopusovary, weak but significant expression was observed in other tissues by RT-PCR analysis. The fact that major expression of the gene occurs in the ovary suggests that it has an important function in this organ.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.