Abstract
Pattern recognition receptors play an important role in insect immune defense. We cloned the β-1,3-glucan recognition protein, lectin-5 and C-type lectin 1 genes of Antheraea pernyi and examined the expression profiles of immune-stimulated pupae. After infection with Bacillus subtilis, Escherichia coli, Antheraea pernyi nuclear polyhedrosis virus (ApNPV) and Saccharomyces cerevisiae, respectively, the pupae showed different gene expression levels in the different tissues examined (midgut, fatbody, epidermis, testis, and hemocytes). ApβGRP and Aplectin-5 was induced by all the microorganisms, and mainly in epidermis and hemocytes, but not in testis; Aplectin-5 was also expressed in fatbody. Ap C-type lectin 1 was, on the contrary, highly expressed in testis and also in fatbody, but not in hemocytes. Unlike ApβGRP and Aplectin-5, Ap C-type lectin 1 was not induced by Gram-positive bacteria. The results suggest that the cloned lectins may have different functions in different tissues of A. pernyi.
Highlights
Lepidopteran immune resistance to foreign pathogenic microorganisms relies on the recognition of conserved microbial structures of the microorganisms
Total RNA was used to clone the fragments of $Sȕ*53, Aplectin-5 and Ap C-type lectin 1 (ApCTL1) cDNA by RT-PCR (TaKaRa)
Pattern recognition receptors play an important role in the insect immune system, they can interact with pathogen-associated molecular patterns (PAMPs) and initiate reactions in the Toll pathways or imd pathways to produce antimicrobial peptides or protease inhibitors for resistance against pathogenic microorganisms [25,26]
Summary
Lepidopteran immune resistance to foreign pathogenic microorganisms relies on the recognition of conserved microbial structures of the microorganisms This leads to downstream reactions: cellular responses including phagocytosis and nodule formation, humoral responses such as antimicrobial peptide synthesis, and melanization after activation of the prophenoloxidase system [1,2]. The C-type lectin superfamily is a major type of pattern recognition receptors, which are calcium-dependent carbohydrate-binding proteins [9,10] They take part in immune responses such as recognition of bacteria and fungi, activation of prophenoloxidase, and hemocyte nodule formation [11±15]. C-type lectin 1 genes of A. pernyi were cloned, and their expression profiles following infection with different microorganism were investigated Their in silico-deduced secondary structures and microorganism recognition specificities are described
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
