CDNA-based comparative genomic hybridization (cCGH) analysis of genomic aberrations of pancreatic cancer cells

Abstract

4122 Introduction: Pancreatic carcinoma (PanCa) is the fourth leading cause of cancer mortality in US with a 5-year survival rate of 5%. urrent treatments have only a minimal survival benefit. In this study, we are using cDNA-based comparative genomic hybridization (cCGH) technologies to search for common genomic alternations in human PanCa cell lines. Methods: Genomic DNA from seven human PanCa cell lines was extracted and labeled with Cyanine 3-dUTP. Normal human genomic DNA isolated from leukocytes of healthy male and female volunteers was labeled with Cyanine 5-dUTP. The labeled probe mixtures were then hybridized with 10K (5K in replicates) cDNA slides prepared by the UT MD Anderson Cancer Genomics Core Laboratory. The gain or loss of individual genes after background subtraction and appropriate normalization. The identified genomic alternations were then verified by florescent in situ hybridization (FISH) analysis in pancreatic cells and biopsy tissues with human BAC clone DNA and the centromere con...