CDNA and lincRNA microarrays: identification of expression patterns related to metastatization in oral squamous cell carcinoma (1048.6)

Abstract

Oral squamous cell carcinoma (OSCC) metastatic cascade still a poorly understood mechanism, in terms of gene expression differences between primary site and metastatic cells at lymph nodes. Using 20 matched samples of OSCC cases, being 10 from primary sites and 10 metastastic lymph nodes we performed lincRNA and cDNA microarrays analysis (Agilent`s Sureprint G3 Human Genome V2.0). Our results showed 617 genes and lincRNAs significantly differentially expressed between metastatic lymph nodes and primary tumors. Integrated pathway analysis showed alteration in canonical pathways such as Ras homolog gene family, member A signaling pathway (RhoA pathway; p=0.002) and tumor necrosis factor receptor 2 signaling pathway (TNFR2 pathway; p=0.009). The most up‐regulated genes included SNORA65 (9,045‐fold), ZMAT1 (4,776‐fold) HNRNPU‐AS1 (4,626‐fold), RNU12 (4,557‐fold), TLE4 (4,555‐fold), and the most down‐regulated included MYBPH (‐67,818‐fold), ACTA1 (‐58,551‐fold), TNNC1 (‐23,119‐fold), F2RL2 (‐17,431‐fold) and MYLPF (‐13,785‐fold). Regarding lincRNAs, MALAT1 was significantly overexpressed in metastatic lymph nodes, with a 4.2‐fold increase. Our study shows the existence of several genes and lincRNAs with different expression patterns between OSCC primary tumor and metastatic lymph nodes, and also points to novel genes, which has not yet been implicated in OSCC metastasis, providing new potential targets in oral cancer research.Grant Funding Source: Supported by FAPESP grants 2011/18606‐7‐0 and 2010/08400‐0