Abstract
Background and AimsThe pathogenesis of inflammatory bowel disease (IBD) has not been fully understood yet. Eosinophils (Eo) are one type of the major proinflammatory cells of the chronic inflammation in the intestine. CD98 is involved in the pathogenesis of a number of inflammations. This study aims to elucidate the role of CD98+ Eos in the initiation of intestinal inflammation.MethodsThe colon biopsies were collected from 60 patients with IBD. The expression of CD98 in the biopsies was examined by immunohistochemistry. The serum levels of the flagellin (FGN) antibody and Eo-derived mediators in the culture supernatants were assessed by enzyme-linked immunosorbent assay. The role of FGN on Eo activation was examined in a cell culture model. The role of FGN in the induction of colitis was observed in a mouse model.ResultsCompared to normal controls, the frequency of CD98+ Eos was markedly increased in the IBD colon mucosa. FGN were detected in the colon biopsies and in the sera of IBD patients. Exposure to FGN induced the expression of galectin 3 (the ligand of CD98) in dendritic cells. The exposure to galectin 3 activated the CD98+ Eos. After treatment with FGN intrarectally, mice with eosinophilia showed severe inflammation in the colon.ConclusionsThe interaction of galectin 3 and CD98 can induce Eos to release chemical mediators that contributes to the initiation of the intestinal inflammation.
Highlights
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the intestine
The interaction of galectin 3 and CD98 can induce Eos to release chemical mediators that contributes to the initiation of the intestinal inflammation
Eos were detected in the non-inflammatory bowel disease (IBD) intestinal mucosa; but the frequency (11.6% 62.2%;) of CD98+ Eos was significantly lower in the nonIBD specimens than that in the IBD intestinal mucosa
Summary
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the intestine. The mediators are involved in the pathogenesis of allergic diseases such as asthma [3]. Eos are involved in the pathogenesis of a number of other disorders, such as rheumatoid arthritis [4], infectious diseases [5] and idiopathic [6] inflammatory disorders. The chemical mediators of Eos are involved in the intestinal inflammation. The mechanism by which Eos induce inflammation in the intestine remains to be further understood. The pathogenesis of inflammatory bowel disease (IBD) has not been fully understood yet. Eosinophils (Eo) are one type of the major proinflammatory cells of the chronic inflammation in the intestine. CD98 is involved in the pathogenesis of a number of inflammations. This study aims to elucidate the role of CD98+ Eos in the initiation of intestinal inflammation
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