CD90(+)CD45(−) intraperitoneal mesothelial-like cells inhibit T cell activation by production of arginase I

Abstract

In this study, we analyzed intraperitoneal cells recovered from human samples and found that CD90(+)CD45(−) cells exist as a minor population but vigorously grow in culture, showing the morphological features of mesothelial cells (MC). Interestingly, the MC highly expressed arginase I and markedly suppressed T cell proliferation with the reduction of CD3 ζ chain expression in T cells stimulated by coated anti-CD3 mAb. The addition of nor-NOHA (500μM), or l-arginine (1mM) mostly restored the inhibitory effect of MC on T cell proliferation as well as the reduced expression of CD3 ζ chain. The expression level of CD3 ζ chain in T cells in the peritoneal cavity was significantly down-regulated from circulating T cells. These results suggest that intraperitoneal free MC have immunomodulatory functions through the control of l-arginine level, and thus may play significant roles in the pathogenesis of various diseases in the peritoneal cavity.