CD8+ T cells in Trypanosoma cruzi chronically infected patients after anti-parasitic treatment

Abstract

Event Abstract Back to Event CD8+ T cells in Trypanosoma cruzi chronically infected patients after anti-parasitic treatment Jose Mateus1, 2*, Paola Lasso1, 2, Nubia Roa3, John M. González4, Concepción Puerta2 and Adriana Cuéllar1* 1 Pontificia Universidad Javeriana, Colombia 2 Pontificia Universidad Javeriana, Colombia 3 Pontificia Universidad Javeriana, Colombia 4 Universidad de los Andes, Colombia CD8+ T cells have been shown to be central in the control of Trypanosoma cruzi infection, the etiological agent of Chagas disease. This cellular compartment is a highly heterogeneous including different phenotypes and functional cellular activities: stem cell memory (TSCM), central memory (TCM), transitional memory (TTM), effector memory (TEM) and terminal effector (TTE) cells. During chronic T. cruzi infection in humans, circulating CD8+ T cells exhibit attenuated effector functions and a degree of terminal differentiation. Recently, we observed a low frequency of circulating CD8+ TSCM cells in chronic chagasic patients (CCPs) with severe heart disease and an increased frequency of inhibitory receptors associated with a decreased functional activity on CD8+ T cells from CCPs. In murine model of T. cruzi infection was shown that anti-parasitic treatment was associated with reduction of the parasitic load and an increased frequency of CD8+ TCM. Therefore, the aim of the present study was to compare circulating CD8+ T cell subsets and the frequency of inhibitory receptors in CCPs without or with anti-parasitic treatment. A total of 18 CCPs without or with anti-parasitic treatment from endemic areas were recruited at the Instituto Nacional de Salud and Hospital Universitario San Ignacio in Bogotá, Colombia. Peripheral blood mononuclear cells (PBMCs) were obtained from all CCPs from heparin anti-coagulated blood and cells were labeled with a panel of conjugated antibodies to characterize the CD8+ T cell subsets (CD45RA, CCR7, CD28, CD27, CD127, CD95) and to evaluate the frequency and expression of inhibitory receptors (PD-1, CTLA-4, TIM-3, CD160, 2B4). It was observed an increased frequency of CD8+ TCM cells and a decreased frequency of CD8+ T cells that express inhibitory receptors in CCPs with anti-parasitic treatment compared to CCPs without anti-parasitic treatment. These findings suggest that anti-parasitic treatment in CCPs allows the restoration of the CD8+ TCM cells and the reverse of cellular mechanism of control (inhibitory receptors) that could be associated with the reestablishment of functional activity. Acknowledgements We thank Rafael Herazo of the Instituto Nacional de Salud for his participation in the recruitment and medical examination of CCPs. We are grateful to the Pontificia Universidad Javeriana for funding the project “Distribución de subpoblaciones de LT CD8+ en pacientes chagásicos” Keywords: Chagas Disease, CD8+ T cell memory, inhibitory receptors, Chronic infection, Trypanosoma cruzi Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Infectious and parasitic diseases Citation: Mateus J, Lasso P, Roa N, González JM, Puerta C and Cuéllar A (2015). CD8+ T cells in Trypanosoma cruzi chronically infected patients after anti-parasitic treatment. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00189 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Apr 2015; Published Online: 14 Sep 2015. * Correspondence: Dr. Jose Mateus, Pontificia Universidad Javeriana, Bogotá, Colombia, jmateust@gmail.com Dr. Adriana Cuéllar, Pontificia Universidad Javeriana, Bogotá, Colombia, acuellar@javeriana.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jose Mateus Paola Lasso Nubia Roa John M González Concepción Puerta Adriana Cuéllar Google Jose Mateus Paola Lasso Nubia Roa John M González Concepción Puerta Adriana Cuéllar Google Scholar Jose Mateus Paola Lasso Nubia Roa John M González Concepción Puerta Adriana Cuéllar PubMed Jose Mateus Paola Lasso Nubia Roa John M González Concepción Puerta Adriana Cuéllar Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.