CD70 Expression on Spodoptera Frugiperda (Sf9) Cells by Baculovirus to Induce CD27 Stimulation in Mouse B Cells

Abstract

Members of the tumor‐necrosis factor receptor (TNFR) superfamily of molecules have a variety of important roles in the regulation of activation and apoptosis of many cell types, but they are particularly important for cells of the immune system as expressed on B cells and T cells. CD27 is expressed by a subpopulation of B cells, and it is thought to be a marker of memory cells. Although signals delivered by CD27 overlap with those delivered by CD40, they seem to be most important in the late stages of the humoral immune response. For achievement of selective TNF‐TNFR stimulation without ¡°noise¡± background stimulation, insect cell line based expression system was adopted. In this study, CD70, which is the ligand of CD27 on B cells and T cells, was expressed on Spodoptera frugiperda (Sf9) cells with Baculovirus expression system to induce CD27‐CD70 interaction. CD27 stimulation by mouse CD70 expressed Sf9 cells, upregulated CD14 and CD80 expression on A20, mouse B cell line. Interestingly, levels of CD40 and IgG expression was decreased after CD27 stimulation on A20 cells. We can apply this insect cell lines to induction of physiologic condition of receptor‐ligand interaction between immune cells.