Abstract
Among the subsets that define hematopoietic stem cells (HSCs), CD34 − c-kit + Sca-1 + lineage marker − (CD34 −KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin β 3) within the CD34 −KSL population. Approximately 60% of CD34 −KSL cells showed high expression of CD61. CD61 HighCD34 −KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61 HighCD34 −KSL cells also contained significantly more LTR-HSCs than CD61 Low/−CD34 −KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker.
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