CD49b regulates the establishment and maintenance of T helper cell memory

Abstract

Event Abstract Back to Event CD49b regulates the establishment and maintenance of T helper cell memory Asami Hanazawa1* and Koji Tokoyoda1* 1 German Rheumatism Research Center Berlin, Germany We have demonstrated that professional memory CD4 T cells reside and rest in the bone marrow (BM) adhering to IL-7-expressing stromal niches. Their absence induces a defective generation of high-affinity long-lived plasma cells. However, the molecular mechanisms on their establishment and maintenance remain unclear. We here demonstrate the roles of CD49b, which is also called integrin alpha2 and is expressed on most memory CD4 T cells, in the establishment and maintenance of memory CD4 T cells in the BM. We first counted antigen-specific effector and memory CD4 T cells in immunized CD49b-deficient and wild-type mice. The number of CD49b-deficient memory CD4 T cells was significantly impaired compared to wild-type cells, while the number of effector cells in both mice was comparable. So, to clarify why CD49b-deficient memory CD4 T cells are not generated, we transferred CD49b-deficient effector CD4 T cells into naive mice and evaluated their migration ability into the BM. The loss of CD49b induced a defective migration of effector cells into the BM. Then, to examine a role of CD49b in the maintenance of memory CD4 T cells, we inhibited the integrin during memory phase with injection of Fab fragments of anti-CD49b neutralization antibodies. The inhibition of CD49b reduced the number of memory CD4 T cells in the BM. We suggest that CD49b regulates the establishment and maintenance of memory CD4 T cells controlling the immigration of memory cell precursors into the BM and also the persistence of memory CD4 T cells on their survival niches. Keywords: T helper cell memory, CD49b, Collagen, niche, Immunological memory, Bone Marrow Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Hanazawa A and Tokoyoda K (2013). CD49b regulates the establishment and maintenance of T helper cell memory. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00827 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Asami Hanazawa, German Rheumatism Research Center Berlin, Berlin, Germany, ahanazawa@ciea.or.jp Dr. Koji Tokoyoda, German Rheumatism Research Center Berlin, Berlin, Germany, tokoyoda@tottori-u.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Asami Hanazawa Koji Tokoyoda Google Asami Hanazawa Koji Tokoyoda Google Scholar Asami Hanazawa Koji Tokoyoda PubMed Asami Hanazawa Koji Tokoyoda Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.