Abstract
Objective: EpsteinBarr virus (EBV) latent membrane protein 1 (LMP1) is known to plays important role in B cells growth and transformation. LMP1 is considered to be a functional homologue of the CD40 receptor and they can activate many overlapping signaling pathways. In this study, we compared the function of CD40 with that of LMP1 in B cells transformation. Materials and methods: Expression of CD40L was observed in infected B cells with LMP1 mutated EBV. To observe the expression reverse transcription-PCR were performed. Results: This expression of CD40L did not support proliferation and transformation of B cell. Even in vitro proliferation and transformation of B cell infected with LMP1 deleted EBV supplemented with CD40L were also not observed. Conclusion: Despite many similarities shared between CD40 and LMP1, CD40-CD40L interaction didnt complement on LMP1 mediated B cells transformation in vitro. DOI: http://dx.doi.org/10.3329/bjms.v11i2.8175 Bangladesh Journal of Medical Science Vol. 11 No. 02 April 2012: 112-116
Highlights
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that infects the vast majority of the world’s adult population .[1]
Mutations in the CD40 and its ligand (CD40L) gene were identified in X-linked hyper IgM syndrome (XHIM), a disease characterized by drastic inhibition in T cell-dependent humoral immune responses . 10,11 CD40 or CD40L mutated mice had showed severe defects in the establishment of B cell memory and very few lymphoblastoid cell lines (LCL) from XHIM B cells upon EBV infection were observed . [7,10 It was found that EBV infection of B cells induces ectopic expression of CD40L 12 and it’s expression could stimulate CD40-mediated growth signals in B cells 10, 13,14 suggesting that EBV-induced CD40L facilitates B cell proliferation
latent membrane protein 1 (LMP1) and CD40 interact with the same tumor necrosis receptor–associated factors (TRAFs) leading to activation of NF?B, c-Jun NH2 terminal kinase (JNK), and p38
Summary
Cd40 doesn’t Complement The Function Of Epstein-barr Virus (Ebv) Latent Membrane Protein 1 In B Cells Transformation. Abstact: Objective: Epstein–Barr virus (EBV) latent membrane protein 1 (LMP1) is known to plays important role in B cells growth and transformation. We compared the function of CD40 with that of LMP1 in B cells transformation. Materials and methods: Expression of CD40L was observed in infected B cells with LMP1 mutated EBV. To observe the expression reverse transcription-PCR were performed. Results: This expression of CD40L did not support proliferation and transformation of B cell. Even in vitro proliferation and transformation of B cell infected with LMP1 deleted EBV supplemented with CD40L were not observed. Conclusion: Despite many similarities shared between CD40 and LMP1, CD40-CD40L interaction didn’t complement on LMP1 mediated B cells transformation in vitro
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