Abstract
In this study, the role of chicken CD4+CD25+ cells during induced immunotolerance was tested. Properties of chicken CD4+CD25+ cells sorted by flow cytometry were analyzed. Results showed that chicken CD4+CD25+ cells express IL-10, TGF-β highly and suppress proliferation of CD4+CD25− cells in vitro. To induce immunotolerance, embryos were inoculated with bovine serum albumin (BSA) via an intravascular route on embryo incubation day 20 (EID20), and after hatching chicks experienced BSA immunization four times at 7-day intervals. Serum anti-BSA antibodies and CD4+CD25+ cell ratio was analyzed. Results showed that humoral tolerance was obtained and the CD4+CD25+ cell percentage in peripheral blood lymphocytes increased along with this progress. Injection of anti-chicken CD25 antibody via an intravascular route on EID16 is applied to block CD4+CD25+ cells, and the CD4+CD25+ cell ratio decreased significantly up to 35 d post-hatch. Based on the above, injections of anti-chicken CD25 antibody on EID16 and BSA on EID20 were carried out sequentially, and tolerance level was contrasted to the BSA-injection group. Data revealed the anti-BSA antibodies increased significantly in the CD4+CD25+ cell-blocked groups indicating that immune tolerance level was weakened. In conclusion, chicken CD4+CD25+ cells are essential in maintaining induced immune tolerance.
Highlights
The poultry bioreactor which can produce foreign proteins based on poultry transgenic technology has great potential economic benefits [1,2]
Found that CD4+ CD25+ cells started to appear at EID15 in thymus
The levels of anti-bovine serum albumin (BSA) IgY, IgM, IgA antibodies in anti-CD25 antibodies-injection prior to BSA-injection group are significantly increased than those in BSA-injection control group. These results indicated that injection of anti-chicken CD25 antibodies prior to BSA injection could weaken the level of the humoral immune tolerance to BSA
Summary
The poultry bioreactor which can produce foreign proteins based on poultry transgenic technology has great potential economic benefits [1,2]. In order to prevent the immune system in avians from recognizing and removing foreign proteins, it is important to induce the immune tolerance and study the mechanisms of tolerance of birds. Chickens are suited for immune tolerance studies because their ontogenesis is different from mammals in that avian embryos develop outside the mother. There have been studies on the methods and mechanisms of immune tolerance induction artificially in chickens. Studies of Tempelis et al [3] and Wolfe et al [4] showed that intraperitoneal or intravenous injections of about 11 mg of bovine serum albumin into newly hatched chicks can induce tolerance. Tolerance was observed after injection of human serum albumin (HSA) into chicken embryo yolk sacs [5] or after hatched [6]. It is not difficult to find that a fairly huge amount of protein antigen
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
