CD16 and CD56 mRNA Expression in Decidua of Patients with Missed and Spontaneous Abortions

Abstract

Study Objective to estimate relative mRNA expression of receptors CD16 and CD56 in decidual tissue of the patients with missed and spontaneous abortions. Design Prospective cohort study. Setting Academic affiliated tertiary care center. Patients or Participants Patients with spontaneous abortions (n=22), missed abortions (n=22) and women with normal pregnancy, admitted for artificial abortions (control group, n=57) were examined at 6-10 weeks of gestation. Patients with severe extragenital diseases, antiphospholipid syndrome, endocrine disorders, uterine malformations, and fetal chromosomal abnormalities were excluded from the research. Interventions Uterine abrasion. Measurements and Main Results Decidual tissue was obtained by uterine abrasion. Relative mRNA expression of CD16 and CD56 was defined by reverse transcription-quantitative polymerase chain reaction (RT-PCR). Peptidylprolyl isomerase А and beta-actin were used as housekeeping genes. Expression of mRNA was counted in relative units by ΔΔCq method. Data were performed as the median and interquartile range and compared using Kruskal-Wallis and Mann-Whitney tests (GraphPad Prism 9.0.2). Relative mRNA expression of CD56 (a marker of natural killer (NK) cells) in decidual tissue of the patients with spontaneous and missed abortions didn't have significant differences with the control group, which confirms the data of meta-analysis (Seshadri S., Sunkara S.K., 2013). Relative mRNA expression of CD16 in decidua of patients with missed abortions (837,53 (313,00; 1082,39)) was lower compared to women with spontaneous abortions (1077 (510,23; 1541,37)) and the control group (1692,57 (1243,34; 2759,13)) (p<0,0001 and p<0,00001, respectively). Expression of CD16 in decidua of the patients with spontaneous abortions was lower, than in the control group (p<0,001). CD16 (FcγRIII) is involved in antibody-dependent cellular cytotoxicity. Uterine NK cells have CD56 + CD16- phenotype (Tang A.W., 2011). The main cells in the decidua expressing CD16 are monocytes, macrophages (Ning F. et al., 2016), and neutrophils (Presicce P. et al., 2018). Conclusion CD16 can play an important role in the pathogenesis of early miscarriages. to estimate relative mRNA expression of receptors CD16 and CD56 in decidual tissue of the patients with missed and spontaneous abortions. Prospective cohort study. Academic affiliated tertiary care center. Patients with spontaneous abortions (n=22), missed abortions (n=22) and women with normal pregnancy, admitted for artificial abortions (control group, n=57) were examined at 6-10 weeks of gestation. Patients with severe extragenital diseases, antiphospholipid syndrome, endocrine disorders, uterine malformations, and fetal chromosomal abnormalities were excluded from the research. Uterine abrasion. Decidual tissue was obtained by uterine abrasion. Relative mRNA expression of CD16 and CD56 was defined by reverse transcription-quantitative polymerase chain reaction (RT-PCR). Peptidylprolyl isomerase А and beta-actin were used as housekeeping genes. Expression of mRNA was counted in relative units by ΔΔCq method. Data were performed as the median and interquartile range and compared using Kruskal-Wallis and Mann-Whitney tests (GraphPad Prism 9.0.2). Relative mRNA expression of CD56 (a marker of natural killer (NK) cells) in decidual tissue of the patients with spontaneous and missed abortions didn't have significant differences with the control group, which confirms the data of meta-analysis (Seshadri S., Sunkara S.K., 2013). Relative mRNA expression of CD16 in decidua of patients with missed abortions (837,53 (313,00; 1082,39)) was lower compared to women with spontaneous abortions (1077 (510,23; 1541,37)) and the control group (1692,57 (1243,34; 2759,13)) (p<0,0001 and p<0,00001, respectively). Expression of CD16 in decidua of the patients with spontaneous abortions was lower, than in the control group (p<0,001). CD16 (FcγRIII) is involved in antibody-dependent cellular cytotoxicity. Uterine NK cells have CD56 + CD16- phenotype (Tang A.W., 2011). The main cells in the decidua expressing CD16 are monocytes, macrophages (Ning F. et al., 2016), and neutrophils (Presicce P. et al., 2018). CD16 can play an important role in the pathogenesis of early miscarriages.