CD147 and Cyclooxygenase Expression in Feline Oral Squamous Cell Carcinoma.

Walaa Nasry,Chelsea Martin,Haili Wang,Wessel Dirksen,Kathleen Jones,Juan Rodriguez-Lecompte,Thomas Rosol

doi:10.3390/vetsci5030072

Abstract

Feline oral squamous cell carcinoma (OSCC) is a highly invasive form of cancer in cats. In human OSCC, cluster of differentiation 147 (CD147) contributes to inflammation and tumor invasiveness. CD147 is a potential therapeutic target, but the expression of CD147 in feline OSCC has not been examined. Immunohistochemistry was used to determine if cyclooxygenase 2 (COX-2) and CD147 expression in feline OSCC biopsies was coordinated. Tumor cells were more likely to express COX-2 (22/43 cases or 51%) compared to stroma (8/43 or 19%) and adjacent oral epithelium (9/31 cases or 29%) (p < 0.05). CD147 was also more likely to occur in tumor cells compared to stroma and adjacent mucosa, with 21/43 (49%) of cases having >50% tumor cells with mild or moderate CD147 expression, compared to 9/28 (32%) in adjacent epithelium and only 5/43 (12%) in adjacent stroma (p < 0.05). In feline OSCC cell lines (SCCF1, SCCF2, and SCCF3), CD147 gene expression was more consistently expressed compared to COX-2, which was 60-fold higher in SCCF2 cells compared to SCCF1 cells (p < 0.05). CD147 expression did not correlate with COX-2 expression and prostaglandin E2 (PGE2) secretion, indicating that they may be independently regulated. CD147 potentially represents a novel therapeutic target for the treatment of feline OSCC and further study of CD147 is warranted.

Highlights

Oral squamous cell carcinoma (OSCC) is the most common tumor in the oral cavity of cats, accounting for 61% of oral cancers [1]
The purpose of this study was to determine if cluster of differentiation 147 (CD147) is expressed in feline oral squamous cell carcinoma (OSCC) biopsy tissues and cell lines, and to determine if CD147 expression is associated with cyclooxygenase 2 (COX-2) expression and prostaglandin E2 (PGE2) synthesis
Replacing the primary antibody with control rabbit IgG eliminated the staining (Figure ??B,D), CD147 immunohistochemistry gave a positive signal in feline small intestine enterocytes, as well as feline OSCC (Figure ??E,G)

Summary

Introduction

Oral squamous cell carcinoma (OSCC) is the most common tumor in the oral cavity of cats, accounting for 61% of oral cancers [1]. Most cats are considered to be senior at the time of diagnosis (average age of 12.5 to 13 years) [3,4] These tumors arise most commonly from the gingiva, tongue, and sublingual region [1,4]. OSCC lesions often appear as small raised masses or areas of ulceration that can go unnoticed by the owner. By the time they are diagnosed, they tend to have already become invasive and are regularly associated with tooth loss and bone destruction when they occur near the mandible or maxilla [5]. Subsequent mortality via elective euthanasia commonly occurs when faced with this invasive disease accompanied by pain and anorexia [3]

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