Abstract
• "Blood-island" like cell clusters is not the only pattern in the emergence of EPCs from MNCs during culturing. • We identified two more distinct attributes, biphasic EPCs and de novo EPCs. • EPCs might coexist as a subset in the MNCs. • CD34 + CD133 + cells from both rabbit and human primary endothelial cell culture can give rise to EPCs. • CD133 is subsequently lost during progression as a cell line, confirming that it is an early cell marker.
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