CD105 (Endoglin) expression in breast carcinoma effusions is a marker of poor survival

Abstract

We analyzed the expression and clinical role of endoglin (CD105) in breast carcinoma effusions. Endoglin levels were measured in 36 effusion supernatants by ELISA and studied for association with the cancer-associated markers calprotectin, VEGF, and the VEGF receptor sFlt1. Endoglin expression was further studied in 46 effusions and 22 primary carcinomas using immunohistochemistry. The four secreted molecules were detected in all specimens and their levels significantly correlated ( p < 0.001). In effusions, endoglin was localized to carcinoma cells and reactive mesothelium using immunohistochemistry. Tumor cell expression was higher in effusions compared to primary carcinomas ( p = 0.025), and in post-chemotherapy compared to pre-chemotherapy effusions ( p = 0.017). Higher tumor endoglin expression was associated with poor overall ( p = 0.021) and disease-free ( p = 0.032) survival in univariate analysis, and was an independent predictor in Cox multivariate analysis ( p = 0.001 and p = 0.038, respectively). Our data suggest that endoglin may be an important therapeutic target in metastatic breast cancer.