CD and NMR determination of the solution structure of a peptide corresponding to T4 lysozyme residues 38–51

Abstract

Solid phase methods have been used to synthesise a peptide corresponding to residues 38–51 of T4 lysozyme. The peptide, LYS(38–51), encompasses helix B in the crystal structure of T4 lysozyme. CD and 1H-NMR analysis showed that the peptide was unstructured in aqueous solution but adopted a helical conformation in the more hydrophobic environment provided by 50% TFE and SDS micelles. The solution structure derived from the NMR data was similar to that of the helix in the X-ray structure, although there was some fraying at the N-terminus.