Abstract

BackgroundChemokine receptors have been suggested to be preferentially expressed on CD4+ T cells with CCR3 and CCR8 linked to the T helper (Th) 2 subset and CCR5 and CXCR3 to the Th1 subset, however this remains controversial.ObjectiveOur aim was to compare the CCR3, CCR5, CCR8 and CXCR3 expression in memory Th cells from allergic, asymptomatically sensitized and healthy individuals.MethodsPeripheral blood mononuclear cells from 8 pollen allergic rhinitis patients, 10 asymptomatically sensitized and 10 healthy individuals were stimulated for 7 days with allergen or tetanus toxoid. CCR3, CCR5, CCR8, CXCR3, CD4 and CD45RO were detected by flow cytometry.ResultsNo differences in chemokine receptor expression were observed between the three groups on day 0, and seven days of unstimulated culture did not change the expression. Both antigenic stimuli increased the chemokine receptor expression, tetanus toxoid being the most potent. No differences in percentage chemokine receptor positive memory Th cells were observed between the three groups on day 7. Only a change in MFI for CCR5 was significantly different between the three groups after allergen stimulation of the Th cells.ConclusionWe conclude that even though allergen and antigen induced increased chemokine receptor expression, no differences in profiles were identified in memory Th cells from patient groups with different atopic status.

Highlights

  • The prevalence of allergy is increasing in the westernized part of the world with estimates suggesting that 20–30% of the population is affected [1]

  • No significant differences in the percentage of CCR3+, CCR5+, CCR8+ and CXCR3+ memory T helper (Th) cells from allergic, asymptomatically sensitized and healthy individuals were observed (Figure 1)

  • Day 7 Effect of stimulation No significant differences in chemokine receptors were observed between day 0 and the cells having been kept in antigen-free medium for 7 days as controls

Read more

Summary

Introduction

The prevalence of allergy is increasing in the westernized part of the world with estimates suggesting that 20–30% of the population is affected [1]. Unlike the reaction of most IgE-sensitized individuals who upon re-exposure to the allergen develop symptoms due to activation and release of mediators from various immune cells, some individuals seem to exhibit an IgE positive phenotype without having any allergic symptoms. These individuals have been described in the literature as asymptomatically sensitized and are phenotypically considered to be a group between the allergic and the healthy individuals with an increased risk of developing allergy [2,3]. Chemokine receptors have been suggested to be preferentially expressed on CD4+ T cells with CCR3 and CCR8 linked to the T helper (Th) 2 subset and CCR5 and CXCR3 to the Th1 subset, this remains controversial

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call