CC and CXC Chemokines Patterns in Psoriasis Determined by Protein Array Method Were Influenced by Goeckerman’s Therapy

David Pohl,Lenka Borská,Zdeněk Fiala,Ctirad Andrýs,Jan Krejsek,Květoslava Hamáková,Karel Ettler

doi:10.14712/18059694.2016.100

David Pohl, Lenka Borská + Show 5 more

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https://doi.org/10.14712/18059694.2016.100

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Abstract

Goeckerman's therapy (GT) of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. The aim of this study was to evaluate the influence of Goeckerman's therapy of psoriasis on the levels of proangiogenic chemokines ENA-78 (CXCL5, Epithelial Cell Derived Neutrophil Attractant-78), GRO alpha (CXCL1, Growth-Related Oncogene), IL-8 (CXCL8, Interleukin-8), MCP-1 (CCL2, Monocyte Chemotactic (Chemoattractant) Protein 1) and RANTES (CCL5, Regulated on Activation of Normal T Cell Expressed and Secreted) in peripheral blood of 22 children's patients with psoriasis. 22 otherwise healthy children serve as a control group. The serum levels of chemokines were determined by commercial membrane protein array technique (RayBiotech, USA). Efficacy of Goeckerman's therapy was delineated by PASI score. Disease activity was significantly diminished by Goeckerman's therapy (p < 0.001). Serum levels of GRO alpha and MCP-1 in patients before GT were significantly higher than those measured in healthy blood donors (GRO alpha: p = 0.0128 and MCP-1: p = 0.0003). Serum levels of GRO alpha, MCP-1 and RANTES were significantly diminished by GT (GRO alpha: p = 0.002, MCP-1: p = 0.048 and RANTES: p = 0.0131). Compared to the healthy controls, serum level of MCP-1 remained significantly increased in psoriasis patients after GT (p < 0.0001). In conclusion, we found that the GT of psoriasis influenced the serum levels of proinflammatory and proangiogenic chemokines, especially GRO alpha, MCP-1 and RANTES. It could be the cause for decreased proangiogenic activity which is described after GT of psoriasis.

Highlights

The original Goeckerman’s method of therapy (GT) of psoriasis, which is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure of the body surface to UV radiation, was introduced by William Goeckerman in 1925 [14]
All results were expressed in Tab. 1 as a means, medians and standard deviations (SD)
Pre-therapy Psoriasis Area and Severity Index (PASI) scores 20.33 ± 9.86 dropped to 7.71 ± 5.22 after therapy (p

Summary

Introduction

The original Goeckerman’s method of therapy (GT) of psoriasis, which is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure of the body surface to UV radiation, was introduced by William Goeckerman in 1925 [14]. In spite of the potentially increased genotoxic risk due to direct exposure of the skin to the poorly defined hydrocarbons present in a coal tar, this therapy is preferred for several reasons. The typical feature of psoriasis is an epidermal hyperproliferation which is closely linked to the high angiogenic activity. This angiogenic activity is firmly established in psoriasis [8, 22]. Intricate process of angiogenesis is carefully regulated by numerous intimate cell-to-cell contacts and humoral interactions involving cytokines, growth and differentiation factors, matrix metalloproteinases, tissue inhibitors of matrix metalloproteinases and chemokines [7, 8]

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