CBF/DREB transcription factor genes play role in cadmium tolerance and phytoaccumulation in Ricinus communis under molybdenum treatments

Abstract

The C-repeat binding factor/dehydration responsive element binding proteins (CBF/DREB) constitute a large group of transcriptional factors. Their role in abiotic stresses such as drought, salinity and low temperature tolerance in plants have been well established, while little information about their role in metals stress tolerance is available. Transcriptomic analyses of four genes (DREB-1A, DREB-1B, DREB-1F and CBF) were carried out in industrially important plant Ricinus communis under cadmium (Cd) and molybdenum (Mo) treatments. Cadmium (in soil) and Mo (as foliar spray) were used separate as well as in combinations. All the genes (except DREB 1A) expressed under Cd stress, while Mo further enhanced their expression. The proline (55.68 ± 5.51 ppm) and phenolic (120.00 ± 14.40 ppm) contents were significant increase in combination treatments of Cd and Mo. Positive and significant correlations of DREB 1B, DREB 1F and CBF genes expressions with free proline (0.92, 0.93 and 0.88 respectively), phenolic (075, 0.77 and 0.62 respectively) contents and Cd accumulation were demonstrated. Nucleotide sequence of R. comunis DREB1F and CBF genes showed more than 80% homology with related genes of other flowering plants. Predicted amino acids sequence of R. communis DREB 1F and CBF protein fragment demonstrated more than 75% homology with related proteins from other flowering plants.