Abstract

Caveolae are small invaginated microdomains located with a variety of signal transduction molecules on the plasma membrane. Recent reports showed that the knockout mice of a muscle-specific protein caveolin-3 (Cav-3 KO), a principal component of the caveolae in heart, displayed an enlargement of ventricular cells. Volume-regulated outwardly rectifying anion channel (VRAC) is activated by membrane stretch, and play a significant role in cell volume regulation in cardiac cells. However, it is unknown the properties of VRAC in the enlarged cardiac cells from Cav-3 KO mice. In this study, we examined that VRAC current and the cell volume regulation in freshly isolated single ventricular cells from Cav-3 KO mice (Hagiwara et al. 2000). Whole-cell current recording showed that the density of VRAC current induced by extracellular hypotonic solution (HYPO) is markedly reduced in the cells from Cav-3 KO mice, compared to that from wild-type (WT) mice. Video-image analysis revealed that the degree of HYPO-induced cell swelling in Cav-3 KO mice is significantly bigger than that in WT mice, and the regulatory volume decrease, which was seen in WT cells after osmotic swelling, is almost lost in cells from Cav-3 KO mice. This result is in parallel with the VRAC inhibition. In contrast, acidic extracellular pH-activated chloride current and extracellular UTP-activated CFTR current were affected less by the deficiency of caveoline-3. The attenuated VRAC current was restored by intracellular application of a VRAC modulator, phosphatidylinositol 3,4,5-trisphosphate (PIP3). These findings suggested that the attenuation of cardiac VRAC current is due to the PIP3 depletion in Cav-3 KO mice.

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