Causes for the High Mortality of European Green Toad Tadpoles in Road Stormwater Ponds: Pollution or Arrival of a New Predator?

Abstract

Declining wetland areas cause many amphibian species to breed inside stormwater ponds (SWPs), which have been constructed alongside major roads to collect and retain polluted road runoff water. However, the suitability of such artificial ponds as a breeding habitat for amphibians remains unclear. Recently, a study found a very low survival rate of European green toad tadpoles (Bufotes viridis) inside SWPs, presumably because of high sediment pollution and/or the presence of a leech Helobdella stagnalis. To establish the effects of sediment pollution and leech presence on tadpole growth and survival, we exposed 480 green toad tadpoles to a number of controlled conditions inside holding tanks. We tested the following conditions: (1) ‘control’ (clean sediment + clean water); (2) ‘leech’ (clean sediment + clean water + leeches); (3) ‘SWP sediment’ (clean water + polluted sediment); and (4) ‘SWP sediment + leech’ (clean water + polluted sediment + leeches). Tadpole size and survival was monitored until metamorphosis and, individuals participated in swim tests and respirometry trials to the test potential effects of pollution on their escape capacity and metabolic rate. We found that the growth rate of tadpoles exposed to the SWP sediment (condition 3) was increased, while pollution had no effect on survival. By contrast, leeches heavily preyed upon tadpoles, leaving no survivors in conditions 2 and 4. Tadpoles swim speed and metabolic rate of toadlets did not differ between the ‘control’ and ‘SWP sediment’ group, the only conditions with surviving individuals. Our study found that leeches had the strongest effect on tadpole survival and were likely responsible for the low survival rates in SWPs observed recently. Hence, we suggest that adequate management measures are needed to limit leech penetration inside SWPs (frequent dredging/draining) to prevent these artificial structures from becoming an ecological trap for locally endangered amphibians but rather a base to help in their recovery.