Cause and effect of N/P ratio decline with eutrophication aggravation in shallow lakes

Abstract

To explore the relationship and cause and effect between eutrophication and the nitrogen (N)/phosphorus (P) ratio, samples from 38 lakes in Wuhan City, China, with differing degrees of eutrophication, were collected for nutrient levels and extracellular enzyme activities (EEA) in the water column from July 2011 to November 2011. The phosphorus fraction, abundance and potential denitrification rate (PDR) as well as community composition of nirS-type denitrifier in sediments of five typical lakes were further analyzed. A higher trophic level index (TSI) corresponded to a lower N/P ratio, which can be attributed to a loss of N and an increase in P. Specifically, in more eutrophic lakes, the enrichment of total organic carbon and all forms of P in sediments could fuel PDR by shaping community composition and increasing the abundance of nirS-type denitrifier as evidenced by correlation and redundancy analysis, ultimately resulting in a loss of N. Meanwhile, iron-bound phosphorus release induced by anoxia and the hydrolysis of organic P accounted for the observed increase of P in the water column. The lower N/P ratio facilitated the production of leucine aminopeptidase, which was unexpectedly induced by high P but not by low N. Similarly, alkaline phosphatase was induced by high N but not by low P. These findings indicate a mutual coupling and interplay between N and P cycling and confirm our hypothesis that P accumulation accelerates N loss in the process of eutrophication.