Causal relationship between OHSS and immune cells: A Mendelian randomization study

Abstract

ObjectiveTo confirm the causal relationship between immune cells and Ovarian Hyperstimulation Syndrome. DesignObtaining data, collecting single nucleotide polymorphisms, detecting instrumental variables heterogeneity, assessing causality, and assessing bidirectional causality. SubjectsA two sample Mendelian study to confirm the causal relationship between immune cells and Ovarian Hyperstimulation Syndrome. ExposureImmune cell phenotype (including 22 million SNPs from GWAS on 3757 European individuals). Main outcome measuresInverse variance weighting, one-sample analysis, MR-Egger, weighted median and weighted mode are used to assess the causal relationship between 731 immunophenotypes and Ovarian Hyperstimulation Syndrome. The weighted median and Mendelian Randomization multi-effect residuals and Mendelian Randomization multi-effect residuals and outlier tests are used to assess bidirectional causality between this two. ResultsAfter False Discovery Rate correction, 9 immunophenotypes were found to be significantly associated with the risk of Ovarian Hyperstimulation Syndrome. B cell panel: IgD+ AC (OR, 0.90) 、CD19 on CD24+ CD27+ (OR, 0.86) 、BAFF-R on CD20- CD38 (OR, −1.22); Mature T cell group panel: EM DN (CD4 -CD8-) AC (OR, 1.46); Myeloid cell panel: Mo MDSC AC (OR, 1.13) 、CD45 on CD33br HLA-DR+ (OR, 0.87); Monocyte panel: HLA-DR on monocyte (OR, 0.86) 、CCR2 on CD14+ CD16+ monocyte (OR, 1.15) 、cDC panel: HLA-DR on myeloid DC (OR, 0.89). ConclusionThis study shows the potential link between OHSS and immune cells by genetic means, providing new ideas for future clinical and basic research.