Abstract
An antibody raised to the gel-purified product (p18) of cauliflower mosaic virus (CaMV) open reading frame (ORF) II was used in conjunction with sensitive serological techniques to detect p18 in inclusion body preparations from turnip tissue infected with the aphid transmissible (AT +) isolate Cabb B-JI. p18 was not detected when tissue was infected with the aphid nontransmissible (AT −) isolate CM4-184 that contains a large deletion in ORF II. However, inclusion body preparations from tissue infected with the AT − isolates Campbell and CM1841 contained p18. Hence, the AT phenotype is not directly correlated with the presence or absence of the ORF II product, as was previously indicated when less sensitive techniques were used for analysis.
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