Cats with single Brugia pahangi infections: relationship between parasitological status and humoral responses to somatic and surface parasite antigens.

Abstract

Cats given a single inoculation of Brugia pahangi infective larvae (L3) were retrospectively allocated into three groups according to parasitological outcome of infection. Recognition of somatic and surface antigens of B. pahangi by sera from each group was compared by ELISA, immunoelectroblotting, and immunoprecipitation techniques. In cats that never became microfilaraemic mean serum IgG antibody levels against somatic extracts from adult male worms, L3, and microfilariae (mf) were higher than levels in cats that initially became microfilaraemic (mf + ve) then spontaneously became nonmicrofilaraemic (mf - ve). The lowest levels of antibody against each stage were found in cats that remained persistently mf + ve. Antigenic components of 18 kD and 22 kD in somatic extracts of adult worms and L3 were recognized by sera from cats that never became mf + ve and by spontaneously mf - ve cats, but not by sera of persistently mf + ve cats. When radioiodinated surface antigens of mixed adult worms and microfilariae were immunoprecipitated by sera from cats in the three groups, no correlation was observed between recognition of individual antigen components and parasitological outcome of infection.