Abstract
In this study, we used a targeted approach to discover single nucleotide polymorphism (SNP) markers in the genome of an endangered primate, Rhinopithecus roxellanae. We first performed polymerase chain reactions using reported comparative anchor tagged sequences (CATS) primers, as well as some of the primers modified according to the Macaca mulatta orthologs. 118 CATS sequences were attainable. Then a denaturing high performance liquid chromatography and sequencing combining method was applied to check single nucleotide polymorphisms within the sequences. A total of 56 original SNPs were successfully developed, which were further proved to be detectable and polymorphism in more individual samples. These SNP markers are expected to be used in genetic researches, such as population structure, demography and local adaptation of this precious species.
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