Cation-dependent light-induced structural changes in visual receptor membranes

Abstract

A stearamide spin probe was used to study the light-induced structural changes in Rod Outer Segment Membranes in the presence of sodium and calcium ions. The correlation time ( τ C) for the reorientation of the probe was calculated in the dark and light. In the presence of sodium ions, τ C = 3.3 × 10 −9 sec in the dark, and 2.7 × 10 −9sec in the light while the opposite was noticed in the presence of calcium ions, τ C = 2.9 × 10 −9 sec in the dark and 3.6 × 10 −9 sec in the light. The correlation times for reorientation of the probe were also calculated in aqueous glycerol solutions of varying viscosities at 20°C. Comparison of the values of τ C (dark and light) suggests a change in local mobility in the ROS corresponding to a macroscopic viscosity difference of approximately 150 cp. The significance of calcium ion interaction with negatively charged groups and the formation of a Schiff base is emphasized.