Cathodic Adsorptive Stripping Voltammetric Determination of the Antitumor Drug Rutin in Pharmaceuticals, Human Urine, and Blood Serum

Abstract

Cathodic adsorptive stripping voltammetry (CASV) was applied for the determination of rutin in pharmaceuticals, human urine, and blood serum. An electrochemical stripping procedure for trace measurements of rutin was developed based on the adsorption of the Cu2+-rutin complex on a hanging mercury drop electrode and applied to the quantification of the drug. Cyclic voltammetry was used to characterize the interfacial and redox behavior of the adsorbed Cu2+-rutin complex. Experimental and instrumental parameters for quantitative determination were optimized, and a detection limit of 4.9 × 10−9 mol · L−1 in presence of Cu2+-ions for quantification of rutin under optimum conditions was derived. The sharp peak of the adsorbed Cu2+-rutin complex associated with an effective interfacial accumulation of this compound facilitates the determination of rutin in biological fluids with good recoveries. The degree of interference from potentially present metal ions and organic compounds on the CASV signal for Cu2+-rutin complex was evaluated.