Abstract
The characteristics of uptake of 3H-noradrenaline (3H-NA) and 3H-adrenaline (3H-A) in the tissues of the sea pansy, Renilla kollikeri, were studied by in vivo incubations. Lineweaver-Burk plots indicated two components of catecholamine accumulation, one representing a high-affinity uptake with an apparent Km of 4.91×10-7 M (3H-NA) or 4.39×10-7 M (3H-A), and the other a low affinity process with an apparent Km of 5.52×10-5 M (3H-NA) or 1.49×10-5 M (3H-A). The high-affinity uptake of both tracers was strongly inhibited at low temperature and in a calcium-free medium, thus suggesting the involvement of a carrier-mediated transport mechanism, but was largely insensitive to sodium omission and ouabain. Accumulations of 3H-NA, but not 3H-A, were highly desipramine-sensitive.
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