Abstract
Inhibition of atherogenesis through inhibition of lipid metabolism has not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done. Inhibition of atherogenesis via inhibition of lipid metabolism can be done through the mechanism of Reverse Cholesterol Transport (RCT). Signaling pathways that play a role in this mechanism is LXR signaling. LXR activation by an LXR agonist led increasing cholesterol efflux. Catechins based on bioinformatics study showed as a potent candidate LXR agonists that can be used as an inhibitor of atherogenesis. This study aims to prove that the administration Catechins green tea Clone GMB4 can prevent atherosclerosis through increasing mechanism cholesterol efflux from macrophage by taking effect of ABCA1, ABCG1, SRB1 gene expression in cultured macrophages were exposed ox-LDL. Long-term goals of the outcome of the research are the use of Catechins Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used as a complementary therapy for the treatment of atherosclerosis and cardiovascular diseases. The research is divided into 5 groups, namely the culture of macrophages without exposed ox-LDL, culture exposed ox-LDL and groups of Catechins dose I, II, III. In vitro study showed that administration of Catechins increases mRNA of ABCA1, whereas mRNA ABCG1 and SRB1 decreased at all three doses given. The result of protein profilling was identified a protein with a molecular weight of 70 kDa by SDS-PGE with silver staining.
Highlights
Cardio vascular disease (CVD) is first cause of death in the world
This study aims to prove that the administration Catechins green tea Clone GMB4 can prevent atherosclerosis through increasing mechanism cholesterol efflux from macrophage by taking effect of ATP-binding cassette subfamily A member 1 (ABCA1), ATP-binding cassette subfamily G member 1 (ABCG1), SRB1 gene expression in cultured macrophages were exposed ox-LDL
Long-term goals of the outcome of the research are the use of Catechins Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used as a complementary therapy for the treatment of atherosclerosis and cardiovascular diseases
Summary
Cardio vascular disease (CVD) is first cause of death in the world. Atherosclerosis is the main contributor cause of death because the trigger of myocardial infarction and ischemic stroke. Liver X receptors act as sensors of cholesterol that works to lower cholesterol levels through increasing the expression of a target gene associated with RCT that are the protein transporter ATP-binding cassette subfamily A member 1 (ABCA1), ATP-binding cassette subfamily G member 1 (ABCG1), Apo A1 and hepatic scavenger receptor class B type I (SRB1) [4]. LXR signaling is activated by an agonist effect on effluks cholesterol in macrophages which can be seen by inspection parameter ABCA1, ABCG1, Apo A1 and SRB-1. Based on the above background research is needed on the role of Catechins in inhibiting atherogenesis through increased effluks cholesterol through the RCT with the ABCA1 gene expression parameter, ABCG1 and SRB1 [6] [7]. “Catechins” is a group of flavonoid consisting of Epicatechin (EC), Epigallocatechin (EGC), Epicatechin Gallate (ECG), Epigallocatechin gallate (EGCG), catechin, gallocatechin, catechin gallate, gallocatechin gallate [11]
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