(±)-Catechin-A Mass-Spectrometry-Based Exploration Coordination Complex Formation with FeII and FeIII.

Lenka Kubicova,Gert Bachmann,Wolfram Weckwerth,Vladimir Chobot

doi:10.3390/cells11060958

Abstract

Catechin is an extensively investigated plant flavan-3-ol with a beneficial impact on human health that is often associated with antioxidant activities and iron coordination complex formation. The aim of this study was to explore these properties with FeII and FeIII using a combination of nanoelectrospray-mass spectrometry, differential pulse voltammetry, site-specific deoxyribose degradation assay, FeII autoxidation assay, and brine shrimp mortality assay. Catechin primarily favored coordination complex formation with Fe ions of the stoichiometry catechin:Fe in the ratio of 1:1 or 2:1. In the detected Fe–catechin coordination complexes, FeII prevailed. Differential pulse voltammetry, the site-specific deoxyribose degradation, and FeII autoxidation assays proved that coordination complex formation affected catechin’s antioxidant effects. In situ formed Fe–catechin coordination complexes showed no toxic activities in the brine shrimp mortality assay. In summary, catechin has properties for the possible treatment of pathological processes associated with ageing and degeneration, such as Alzheimer’s and Parkinson’s diseases.

Highlights

Mass spectrometry has been used for many decades as an effective tool for investigating inorganic and organic compounds
Mass spectrometry, when combined with electroanalytical or other analytical methods, can provide valuable information about chemical properties of substances that can participate in various cellular physiological processes [4–6]
This combination of methods has appeared helpful in formulating hypotheses in cases of hormesis, redox homeodynamic equilibrium, and other biological phenomena [4,5,7]

Summary

Introduction

Mass spectrometry has been used for many decades as an effective tool for investigating inorganic and organic compounds. Despite being a traditional physico-chemical method, mass spectrometry has recently been used in the development of various modern methodologies in biology, such as proteomics [1] and metabolomics [2,3]. Mass spectrometry, when combined with electroanalytical or other analytical methods, can provide valuable information about chemical properties of substances that can participate in various cellular physiological processes [4–6]. This combination of methods has appeared helpful in formulating hypotheses in cases of hormesis, redox homeodynamic equilibrium (homeostasis), and other biological phenomena [4,5,7]. Iron cellular concentrations are carefully controlled, and iron ions are liganded with various storage molecules, such as ferritin or transferrin [15,16]

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Conclusion