(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

E.C.B Silva,M.M.P Guerra,P.C Soares,J.I.T Vieira,L.C.P Arruda

doi:10.1590/1678-4162-10539

E.C.B Silva, M.M.P Guerra + Show 3 more

Open Access

https://doi.org/10.1590/1678-4162-10539

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Abstract

ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P<0.05) with 15µM than with 50 and 100; WOB was lower (P<0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P<0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P<0.05) with0 and 15µM than with50 and 75; LIN was lower (P<0.05) with75 and100µM than with0 and 15; WOB was higher (P<0.05) with0 and 15µM; and PMi was greater (P<0.05) with100µM than 0. Thus, (+)-catechin or EGCG at higher concentrations inhibits the kinematics of frozen goat sperm, in a transitory way, and 100µM of EGCG preserves the PMi.

Highlights

The catechin isomers, (+)-catechin and epigallocatechin gallate (EGCG), are flavanols from the flavonoid family, found in plant-based foods and beverages (Weinreb et al, 2009; Li, 2011)
Semen cryopreservation is a reproductive biotechnique that has an important role in the animal industry, contributing to the expansion of other reproductive techniques (Barbas and Mascarenhas, 2009), and intensifying the productive system and genetic improvement (Leboeuf et al, 2000)
Mammalian sperm cell membranes are susceptible to oxidants attack, once that are rich in polyunsaturated fatty acids (Bansal and Bilaspuri, 2011).the aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)epigallocatechin gallate on goat semen freezability

Summary

Introduction

The catechin isomers, (+)-catechin and epigallocatechin gallate (EGCG), are flavanols from the flavonoid family, found in plant-based foods and beverages (Weinreb et al, 2009; Li, 2011). Semen cryopreservation is a reproductive biotechnique that has an important role in the animal industry, contributing to the expansion of other reproductive techniques (Barbas and Mascarenhas, 2009), and intensifying the productive system and genetic improvement (Leboeuf et al, 2000) During this process, especially during freezing-thawing, the sperm cell is frequently injured (Leboeuf et al, 2000), with consequent reductions in its viability and function (Bansal and Bilaspuri, 2011). Mammalian sperm cell membranes are susceptible to oxidants attack, once that are rich in polyunsaturated fatty acids (Bansal and Bilaspuri, 2011).the aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)epigallocatechin gallate on goat semen freezability

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