Abstract
Accurate interpretation of recruitment rate measurements of microscale particles, such as cells and microbeads, to biofunctional surfaces is difficult because factors such as uneven ligand distributions, particle collisions, variable particle fluxes, and molecular-scale surface separation distances obfuscate the ability to link the observed particle behavior with the governing nanoscale biophysics. We report the development of a hydrodynamically conditioned micropattern catch strip assay to measure microparticle recruitment kinetics. The assay exploited patterning within microfluidic channels and the mechanostability of selectin bonds to create reaction geometries that confined a microbead flux to within 200 nm of the surface under flow conditions. Systematic control of capillary action enabled the creation of homogeneous or gradient ligand distributions. The method enabled the measurement of particle recruitment rates (keff, s-1) that were primarily determined by the interaction of the biomolecular pair being investigated. The method is therefore well suited for relative measurements of delivery vehicle and cellular recruitment potential as governed by surface-bound molecules.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.