Catch and release of concanavalin A by a mannose-immobilized photoaffinity PEGA resin coupled with a cleavable disulfide linker.

Abstract

A photoaffinity PEGA resin containing mannose as a ligand and disulfide as a cleavable linker was prepared. The resin was crosslinked to concanavalin A, a binding protein of mannose, by UV irradiation, and the protein was subsequently released by cleavage of the disulfide linker.