Abstract
The oxidation of putrescine and spermidine were studied in embryogenic and nonembryogenic cell cultures ofPicea abies(L.) Karst., with [1,4‐14C]‐putrescine and [1,4‐14C]‐spermidine as substrates. Activities of putrescine and spermidine oxidation varied at every developmental stage in both cultures. Putrescine was oxidized ca 5 times as fast both in embryogenic and non‐embryogenic tissue as spermidine. Diamine and especially polyamine oxidase activity increased markedly in both tissues towards the end of the culturing. In maturing embryos and in ageing non‐embryogenic cultures, enzyme activities were lower than in non‐differentiated embryogenic calli. Aminoguanidine (1 mM) inhibited di‐ and polyamine oxidation in non‐embryogenic tissue by >60% and >30%, respectively. The pH optimum for putrescine oxidation was 8.0, but in non‐embryogenic tissue spermidine was degraded even more actively at pH 5.0. [14C]‐Spermidine was catabolized to [14C]‐putrescine. Pyrroline dehydrogenase activity was observed in non‐embryogenic spruce tissue cultures.
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