Catabolism of Mannosidase and Fucosidase in Cholesteatoma

Abstract

Objective: To determine the level of glycoconjugates catabolism by marking α-mannosidase (MAN) and α-fucosidase (FUC) activity in cholesteatoma and serum. Method: Middle ear cholesteatomas (Chol), normal skin specimens (C) and serum of cholesteatoma patients and serum from healthy volunteers were included in the study. The concentration of lysosomal exoglycosidases activity was assessed on the basis of p-nitrophenol release from the derivatives of the substrate (MAN from mannose and FUC from fucose). Results: Mean concentration of MAN activity in Chol was 8.31112 pKat/mL in comparison with C (2.621048 pKat/mL). Pearson correlation (MAN activity) was r = 0.26321. Mean activity concentration of FUC in Chol was 10.03288 pKat/mL in comparison with C: 2.723152 pKat/mL. The average concentration of MAN activity in serum from cholesteatoma patients was 3.05712 pKat/mL, compared to the concentration of MAN activity in the control serum 2.73244 pKat/mL. The average concentration of FUC activity in serum of cholesteatoma patients was 5.40336 pKat/mL and significantly higher in comparison with the FUC activity concentrations in C 3.51976 pKat/mL. Conclusion: There is an increase of glycocojugates catabolism due to the significantly higher concentration of MAN and FUC activity in cholesteatoma. The increased concentration of MAN and FUC activity in serum of cholesteatoma patients might suggest the role of those enzymes in the pathogenesis of chronic otitis media with cholesteatoma.