Abstract
Exposure to lead in drinking water elevates blood lead levels and causes permanent impairment to human body. To provide a preventive measure for lead intoxication, here we report a sensitive visual quantification of lead in drinking water and whole blood. GR-5 DNAzyme can be cleaved by Pb2+, releasing an oligonucleotide T that triggers catalytic assembly of hairpin complex H1H2 connecting magnetic microparticles (MMPs) and polystyrene microparticles (PMPs). After loading the particle solution into a capillary-driven microfluidic device, MMPs-H1H2-PMPs are first attracted and removed by a magnetic separator, and the remaining free PMPs continue flowing along the microchannel until accumulating at a particle dam. As such, more lead ions cause shorter PMP accumulation quantifiable by the naked eye. The method achieved a limit of detection (LOD) of 246 pM, and is extremely selective (> 40,000 folds to other metal ions), and highly tolerant to acidity/basicity (6 ‒ 8.5) and water hardness (55 ‒ 318.3 mg/L). More importantly, high recovery rate (> 78%) in tap water and LOD of 2.57 nM for lead level in whole blood were achieved, demonstrating a visual quantification method for screening water safety and lead intoxication with user-friendly interface.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
