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Abstract
The studies reported here demonstrate that immunocompetent lymphoid cells from allogeneic donor guinea pigs stimulate the synthesis of anti-DNP and anti-OVA antibodies by recipients previously primed with DNP-OVA. This allogeneic effect occurs spontaneously in the absence of any further anti-genic challenge. Furthermore, the transfer of allogeneic cells prepares DNP-OVA-primed recipients for a striking secondary anti-DNP response to DNP-BGG; this occurs in equal degree whether or not the cells are derived from BGG-primed donors. We suggest that the allogeneic cells function by virtue of a specific immunologic attack of grafted cells on host cells. This conclusion is made on the basis of the following evidence: (a) The failure of observing the phenomenon with L(2)C leukemia cells and irradiated strain 2 lymph node and spleen cells which, although capable of initiating a host-versus-graft response, are incapable of mediating graft-versus-host reactions; and (b) the inability of (strain 2 x strain 13) F(1) hybrids to mediate the allogeneic effect in strain 13 recipients. The analysis of this phenomenon may offer a key to the delineation of mechanisms involved in the activation of precursors of antibody-forming cells.
Highlights
We report that immunocompetent lymphoid cells from allogeneic donor guinea pigs stimulate a considerable synthesis of antiDNP and anti-OVA antibodies by recipients previously primed with DNP-OVA, in the absence of any further antigenic challenge, eliciting thereby a nonspecific anamnestic response
In order to choose between these two possibilities, we studied the capacity of irradiated strain 2 lymphoid cells, of strain 2 leukemia cells, and of (2 X 13)F1 hybrid lymphoid cells to mediate the allogeneic effect in D N P - O V A - primed strain 13 recipients
The studies reported here demonstrate that immunocompetent lymphoid cells from allogeneic donor guinea pigs stimulate the synthesis of anti-DNP
Summary
The proteins and other reagents used here are identical with those described in the first paper of this series [4]. Immunizations.-Adult inbred strain 2 and strain 13 guinea pigs weighing250400 g were obtained from the Animal Production Section, National Institutes of Health, Bethesda, Md. Primary immunization of recipients was carried out with 1.0 mg of I)NPT-OVAin saline, intraperitoneally, on 3 successivedays. 1.0 mg of I)NP-B GG in saline was used for secondary challenge of recipient guinea pigs. This was administered as a 200 #g intradermal dose followed hr later by an 800 /zg intraperitoneal dose. In the case of strain 13 recipients, dlphenhydramine hydrochloride (Benadryl, Parke, Davis & Co., Detroit, Mich.), 5.0 mg/kg, was administered intramuscularly 1 hr before the intraperitoneal dose of DNP-BGG as a prophylactic measure against anaphylaxis. Animals were bled just beforethe secondary challengeand 4, 7, and 11 days later; antibody determinations were performed as described below
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